Journal: International Journal of Molecular Sciences

Article Title: Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

doi: 10.3390/ijms21176326

Figure Lengend Snippet: Genomic DNA (16S rDNA) of non-transformed E. coli proliferated in ampicillin-free medium (DH5α Amp(−)) and E. coli transformed with a plasmid conferring ampicillin resistance, selected in ampicillin-containing agar medium and proliferated in ampicillin-free medium (pPRO-EX-CAT DH5α Amp(−)), was degraded by DBD plasma torch treatment. A suspension of non-transformed E. coli proliferated in ampicillin-free medium (DH5α Amp(−)) and E. coli transformed with an ampicillin resistance gene-containing pPRO-EX-CAT plasmid, selected in ampicillin-containing agar medium and proliferated in ampicillin-free liquid medium (pPRO-EX-CAT DH5α Amp(−)), was exposed to a DBD plasma torch for the indicated time (min). Viable cell count (colony forming units (CFU)/mL) was then determined before and after treatment. Bands corresponding to amplified E. coli 16S rDNA are indicated by arrowheads. Bands corresponding to a DNA size ladder (M) are labelled on the right-hand side of the gel.

Article Snippet: E. coli (DH5α Competent Cells, Takara Bio, Shiga, Japan) was used for the plasma treatment.

Techniques: Transformation Assay, Plasmid Preparation, Cell Counting, Amplification

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Production of Galactooligosaccharides by a Novel β-Galactosidase of Lactobacillus bulgaricus

doi: 10.3390/ijms232214308

Figure Lengend Snippet: Overexpression and purification of recombinant β-galactosidase of L. bulgaricus 43 in E. coli BL21(DE3), demonstrated by SDS-PAGE in 10% separating gel after silver staining. Legend: (1) Perfect TM Tricolor Protein Ladder; (2) crude extract of the cells of untransformed E. coli BL21(DE3) as control; (3) crude extract from E. coli BL21(DE3) cells, bearing pET-41-β-gal and induced with 1 mM IPTG for 24 h; (4) the purified enzyme β-galactosidase.

Article Snippet: E. coli HST08 strain (STELLAR TM competent cells, genotype F-, endA1 , supE44 , thi-1 , recA1 , relA1 , gyrA96 , phoA , F80d lacZD M15 , D(lacZYA-argF) U169 , D(mrrhsdRMS-mcrBC ), DmcrA , λ-) was purchased from Clontech Laboratories, Inc., A Takara Bio Company (Mountain View, CA, USA).

Techniques: Over Expression, Purification, Recombinant, SDS Page, Silver Staining